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anti kir6 1  (Alomone Labs)


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    Structured Review

    Alomone Labs anti kir6 1
    Anti Kir6 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kir6+1/Anti-Kir6%2E1+Antibody/pm41690912-150-24-25
    Average 94 stars, based on 9 article reviews
    anti kir6 1 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Oral administration of the K ATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis
    Article Snippet: Cultures were blocked in phosphate buffered saline (PBS) solution containing 10% donkey serum (Sigma-Aldrich, St. Louis, MO, USA) and 1% bovine serum albumin (BSA) (VWR International Ltd, UK) for 20 minutes. .. Cells were then incubated with primary antibodies anti-Kir6.1 and anti-Kir6.2 (1:300 dilution, Alomone, Jerusalem, Israel), anti-CD11b (1:500 dilution, Serotec, Oxford, England, UK) at 4°C overnight, followed by secondary antibodies Alexa ® 488 and 596 (1:500, Molecular Probes, Invitrogen, Eugene, OR, USA) for 1 h in blocking solution. .. Slides were mounted in ProLong Gold antifade medium (Molecular Probes, Invitrogen, Eugene, OR, USA) and images were acquired by SP1 confocal microscope (Leica Microsystems GmbH, Wetzlar, Germany), located at the Institut de Biologia Molecular de Barcelona, Microscopy Unit, Parc Científic de Barcelona, Barcelona, Spain.

    Article Title: K ATP channel subunits in rat dorsal root ganglia: alterations by painful axotomy
    Article Snippet: Subsequently DRG embedded in Tissue-Tek ® OCT, were sectioned (5 or 12 μm) with a Leica cryostat (Jung CM 1800; Vienna, Austria), plated onto Gatenby's solution subbed slides, and post-fixed in 4% PFA with 4% sucrose. .. After blocking with 4% normal goat serum for 1 h at room temperature, slides were incubated in polyclonal rabbit antibodies to Kir6.1, Kir6.2 (1:500, Alomone, Jerusalem, Israel), and SUR1 or SUR2 (1:50, Santa Cruz Biotechnology, Santa Cruz, CA). .. Some samples were also co-labeled with antibodies against neurofilament 200 (NF 200; 1:1000, Abcam, Cambridge, UK) and calcitonin-gene related peptide (CGRP; 1:50; Santa Cruz).

    Article Title: Role of Kir6.2 subunits of ATP-sensitive potassium channels in endotoxemia-induced cardiac dysfunction
    Article Snippet: .. The blots were then incubated for 2 h at 25°C with primary antibodies for Kir6.1 (1:200; Alomone Labs Ltd, Israel), and then incubated with IRDye 800CW-conjugated goat anti-rabbit secondary antibody (1:5,000; Rockland, USA) for 1 hour at 25°C. .. The infrared fluorescence image was obtained using Odyssey infrared imaging system (Li-Cor Bioscience, Lincoln, NE), and the band were quantified by Image J software (NIH, USA).

    Blocking Assay:

    Article Title: Oral administration of the K ATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis
    Article Snippet: Cultures were blocked in phosphate buffered saline (PBS) solution containing 10% donkey serum (Sigma-Aldrich, St. Louis, MO, USA) and 1% bovine serum albumin (BSA) (VWR International Ltd, UK) for 20 minutes. .. Cells were then incubated with primary antibodies anti-Kir6.1 and anti-Kir6.2 (1:300 dilution, Alomone, Jerusalem, Israel), anti-CD11b (1:500 dilution, Serotec, Oxford, England, UK) at 4°C overnight, followed by secondary antibodies Alexa ® 488 and 596 (1:500, Molecular Probes, Invitrogen, Eugene, OR, USA) for 1 h in blocking solution. .. Slides were mounted in ProLong Gold antifade medium (Molecular Probes, Invitrogen, Eugene, OR, USA) and images were acquired by SP1 confocal microscope (Leica Microsystems GmbH, Wetzlar, Germany), located at the Institut de Biologia Molecular de Barcelona, Microscopy Unit, Parc Científic de Barcelona, Barcelona, Spain.

    Article Title: K ATP channel subunits in rat dorsal root ganglia: alterations by painful axotomy
    Article Snippet: Subsequently DRG embedded in Tissue-Tek ® OCT, were sectioned (5 or 12 μm) with a Leica cryostat (Jung CM 1800; Vienna, Austria), plated onto Gatenby's solution subbed slides, and post-fixed in 4% PFA with 4% sucrose. .. After blocking with 4% normal goat serum for 1 h at room temperature, slides were incubated in polyclonal rabbit antibodies to Kir6.1, Kir6.2 (1:500, Alomone, Jerusalem, Israel), and SUR1 or SUR2 (1:50, Santa Cruz Biotechnology, Santa Cruz, CA). .. Some samples were also co-labeled with antibodies against neurofilament 200 (NF 200; 1:1000, Abcam, Cambridge, UK) and calcitonin-gene related peptide (CGRP; 1:50; Santa Cruz).

    Membrane:

    Article Title: Functional expression of inwardly rectifying and ATP‐sensitive potassium channels in human pulmonary artery smooth muscle and endothelial cells
    Article Snippet: Enzymes including papain, neutral protease, elastase and collagenase I were from Worthington, and PNU‐37883A from Tocris (Bristol, UK). .. Primary antibodies against Kir2.1, Kir2.2 and Kir6.1 were from Alomone Labs; SUR2 antibody and the membrane marker WGA from Thermo Fisher Scientific (Invitrogen); and CD31 and α‐smooth muscle actin from Sigma‐Aldrich. .. Secondary antibodies, Alexa Fluor 564 goat anti‐rabbit IgG and 488 goat anti‐mouse IgG were also from Thermo Fisher Scientific (Invitrogen).

    Article Title: Functional expression of inwardly rectifying and ATP‐sensitive potassium channels in human pulmonary artery smooth muscle and endothelial cells
    Article Snippet: Enzymes including papain, neutral protease, elastase and collagenase I were from Worthington, and PNU-37883A from Tocris (Bristol, UK). .. Primary antibodies against Kir2.1, Kir2.2 and Kir6.1 were from Alomone Labs; SUR2 antibody and the membrane marker WGA from Thermo Fisher Scientific (Invitrogen); and CD31 and α-smooth muscle actin from Sigma-Aldrich. .. Secondary antibodies, Alexa Fluor 564 goat anti-rabbit IgG and 488 goat anti-mouse IgG were also from Thermo Fisher Scientific (Invitrogen).

    Marker:

    Article Title: Functional expression of inwardly rectifying and ATP‐sensitive potassium channels in human pulmonary artery smooth muscle and endothelial cells
    Article Snippet: Enzymes including papain, neutral protease, elastase and collagenase I were from Worthington, and PNU‐37883A from Tocris (Bristol, UK). .. Primary antibodies against Kir2.1, Kir2.2 and Kir6.1 were from Alomone Labs; SUR2 antibody and the membrane marker WGA from Thermo Fisher Scientific (Invitrogen); and CD31 and α‐smooth muscle actin from Sigma‐Aldrich. .. Secondary antibodies, Alexa Fluor 564 goat anti‐rabbit IgG and 488 goat anti‐mouse IgG were also from Thermo Fisher Scientific (Invitrogen).

    Article Title: Functional expression of inwardly rectifying and ATP‐sensitive potassium channels in human pulmonary artery smooth muscle and endothelial cells
    Article Snippet: Enzymes including papain, neutral protease, elastase and collagenase I were from Worthington, and PNU-37883A from Tocris (Bristol, UK). .. Primary antibodies against Kir2.1, Kir2.2 and Kir6.1 were from Alomone Labs; SUR2 antibody and the membrane marker WGA from Thermo Fisher Scientific (Invitrogen); and CD31 and α-smooth muscle actin from Sigma-Aldrich. .. Secondary antibodies, Alexa Fluor 564 goat anti-rabbit IgG and 488 goat anti-mouse IgG were also from Thermo Fisher Scientific (Invitrogen).

    Whole Genome Amplification:

    Article Title: Functional expression of inwardly rectifying and ATP‐sensitive potassium channels in human pulmonary artery smooth muscle and endothelial cells
    Article Snippet: Enzymes including papain, neutral protease, elastase and collagenase I were from Worthington, and PNU‐37883A from Tocris (Bristol, UK). .. Primary antibodies against Kir2.1, Kir2.2 and Kir6.1 were from Alomone Labs; SUR2 antibody and the membrane marker WGA from Thermo Fisher Scientific (Invitrogen); and CD31 and α‐smooth muscle actin from Sigma‐Aldrich. .. Secondary antibodies, Alexa Fluor 564 goat anti‐rabbit IgG and 488 goat anti‐mouse IgG were also from Thermo Fisher Scientific (Invitrogen).

    Article Title: Functional expression of inwardly rectifying and ATP‐sensitive potassium channels in human pulmonary artery smooth muscle and endothelial cells
    Article Snippet: Enzymes including papain, neutral protease, elastase and collagenase I were from Worthington, and PNU-37883A from Tocris (Bristol, UK). .. Primary antibodies against Kir2.1, Kir2.2 and Kir6.1 were from Alomone Labs; SUR2 antibody and the membrane marker WGA from Thermo Fisher Scientific (Invitrogen); and CD31 and α-smooth muscle actin from Sigma-Aldrich. .. Secondary antibodies, Alexa Fluor 564 goat anti-rabbit IgG and 488 goat anti-mouse IgG were also from Thermo Fisher Scientific (Invitrogen).

    Concentration Assay:

    Article Title: Expression and influence of KATP in umbilical artery smooth muscle cells of patients with hypertensive disorders of pregnancy
    Article Snippet: .. The primary antibody concentration was GAPDH (Bioworld, China) 1:10,000, Kir6.1 (Alomone, Israel) 1:400, SUR2B (Abcam, UK) 1:500. ..



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